IMT

Institute of Molecular Biology and Tumor Research

Prof. Dr. Guntram Suske

Publications





Völkel S, Stielow B, Finkernagel F, Berger D, Stiewe T, Nist A, Suske G. (2018). Transcription factor Sp2 potentiates binding of the TALE homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y to composite genomic sites. J. Biol. Chem. 293:19250-19262.

Stielow, B., Finkernagel, F., Stiewe, T., Nist, A., and Suske, G. (2018). MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6. PLOS Genet. 14, e1007193.

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Wegener, A., Kuspert, M., Sock, E., Philipsen, S., Suske, G., and Wegner, M. (2017). Sp2 is the only glutamine-rich specificity protein with minor impact on development and differentiation in myelinating glia. J. Neurochem. 140, 245-256.

Suske, G. (2017). NF-Y and SP transcription factors – New insights in a long-standing liaison. Biochim. Biophys. Acta 1860, 590-597.

Völkel, S., Stielow, B., Finkernagel, F., Stiewe, T., Nist, A., and Suske, G. (2015). Zinc Finger Independent Genome-Wide Binding of Sp2 Potentiates Recruitment of Histone-Fold Protein Nf-y Distinguishing It from Sp1 and Sp3. PLoS Genet. 11, e1005102.

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Meinders, M., Kulu, D.I., van de Werken, H.J., Hoogenboezem, M., Janssen, H., Brouwer, R.W., van Ijcken, W.F., Rijkers, E.J., Demmers, J.A., Krüger, I., van den Berg, T.K., Suske, G., Gutierrez, L., Philipsen, S. (2015). Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function. Blood, 125, 1957-1967.

Stielow C., Stielow B., Finkernagel F., Scharfe, M. Jarek M. and Suske G. (2014). SUMOylation of the polycomb group protein L3MBTL2 facilitates repression of its target genes. Nucleic Acids Res., 42, 3044-3058.

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Diezko R. and Suske G. (2013). Ligand Binding Reduces SUMOylation of the Peroxisome Proliferator-activated Receptor γ (PPARy) Activation Function 1 (AF1) Domain. PLoS ONE, 8, e66947.

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Terrados, G., Finkernagel, F., Stielow, B., Sadic, D., Neubert, J., Herdt, O., Krause, M., Scharfe, M., Jarek, M., and Suske, G. (2012). Genome-wide localization and expression profiling establish Sp2 as a sequence-specific transcription factor regulating vitally important genes. Nucleic Acids Res. 40, 7844-7857.

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Ingram, R.M., Valeaux, S., Wilson, N., Bouhlel, M.A., Clarke, D., Kruger, I., Kulu, D., Suske, G., Philipsen, S., Tagoh, H., and Bonifer, C. (2011). Differential regulation of sense and antisense promoter activity at the Csf1R locus in B cells by the transcription factor PAX5. Exp. Hematol., 39, 730-740

Stielow B., Krüger I., Diezko R., Finkernagel F., Gillemans N., Kong-a-San J., Philipsen S. and Suske G. (2010). Epigenetic Silencing of Spermatocyte-specific and Neuronal Genes by SUMO Modification of the Transcription Factor Sp3. PLoS Genet 6, e1001203

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Baur, F., Nau, K., Sadic, D., Allweiss, L., Elsässer, H.-P., Gillemans, N., de Wit, T., Krüger, M., Vollmer, M., Philipsen, S.  and Suske G.,  (2010). Specificity Protein 2 (Sp2) is Essential for Mouse Development and Autonomous Proliferation of Mouse Embryonic Fibroblasts. PLoS ONE 5, e9587

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Stielow, B., Sapetschnig, A., Wink, C., Krüger, I., Suske, G. (2008). SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing. EMBO Rep. 9, 899-906.

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Stielow, B., Sapetschnig, A., Krüger, I., Kunert, N., Brehm, A., Boutros, M., and Suske, G. (2008). Identification of SUMO-dependent Chromatin-associated Transcriptional Repression Components by a Genome-wide RNA Interference Screen. Mol. Cell 29, 742-754.

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Van Loo, P. F., Mahtab, E. A., Wisse, L. J., Hou, J., Grosveld, F., Suske, G., Philipsen, S., and Gittenberger-de Groot, A. C. (2007). Transcription Factor Sp3 knockout mice display serious cardiac malformations. Mol. Cell. Biol. 27, 8571-8582.

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Krüger, I., Vollmer M., Simmons, D.G., Elsässer, H.-P., Philipsen, S., and Suske, G. (2007). Sp1/Sp3 compound heterozygous mice are not viable: Impaired erythropoiesis and severe placental defects. Dev. Dyn. 236, 2235-2244

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Yao, D, Taguchi, T., Matsumura, T., Pestell, R., Edelstein, D., Giardino, I, Suske, G., Rabbani, N., Thornalley, P.J., Sarthy, V.P., Hammes, H.P. and Brownlee, M. (2007). High Glucose Increases Angiopoietin-2 Transcription in Microvascular Endothelial Cells through Methylglyoxal Modification of mSin3A. J. Biol. Chem., 282, 31038-31045

Das, S., Ward, S. V., Tacke, R. S., Suske, G., and Samuel, C. E. (2006). Activation of the RNA-dependent protein kinase PKR promoter in the absence of interferon is dependent upon Sp proteins. J. Biol. Chem. 281, 3244-3253.

Rojo, A. I., Salina, M., Salazar, M., Takahashi, S., Suske, G., Calvo, V., de Sagarra, M. R., and Cuadrado, A. (2006). Regulation of heme oxygenase-1 gene expression through the phosphatidylinositol 3-kinase/PKC-zeta pathway and Sp1. Free Radic. Biol. Med. 41, 247-261.

Suske, G., Bruford, E., and Philipsen, S. (2005). Mammalian SP/KLF transcription factors: bring in the family. Genomics 85, 551-556.

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Sapetschnig, A., Koch, F., Rischitor, G., Mennenga, T., and Suske, G. (2004). Complexity of translationally controlled transcription factor Sp3 isoform expression. J. Biol. Chem. 279, 42095-42105.

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Meissner M, Stein M, Urbich C, Reisinger K, Suske G, Staels B, Kaufmann R, Gille J. (2004). PPARalpha activators inhibit vascular endothelial growth factor receptor-2 expression by repressing Sp1-dependent DNA binding and transactivation. Circ Res. 94, 324-332.

Kramps, C., Strieder, V., Sapetschnig, A., Suske, G. and Lutz, W. (2004). E2F and Sp1/Sp3 synergize but are not sufficient to activate the MYCN gene in neuroblastomas. J. Biol. Chem. 279, 5110-5117.

Van Loo, P.F., Bouwman, P., Ling, K.-W., Middendorp, S., Suske, G., Grosveld, F., Dzierzak, E., Philipsen, S. and Hendriks, R.W. (2003).
Impaired hematopoiesis in mice lacking the transcription factor Sp3. Blood 102, 858-866.

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Lagger, G., Doetzlhofer, A., Schuettengruber, B., Haidweger, E., Simboeck, E., Tischler, J., Chiocca, S., Suske, G., Rotheneder, H., Wintersberger, E. and Seiser, C. (2003). The tumor suppressor p53 and the deacetylase HDAC1 are antagonistic regulators of thecyclin-dependent kinase inhibitor p21/WAF1/CIP1 gene. Mol. Cell. Biol. 23, 2669-2679.

Chadjichristos C, Ghayor C, Kypriotou M, Martin G, Renard E, Ala-Kokko L, Suske G, de Crombrugghe B, Pujol JP, Galera P. (2003). Sp1 and Sp3 transcription factors mediate interleukin-1 beta down-regulation of human type II collagen gene expression in articular chondrocytes. J. Biol. Chem. 278, 39762-39772.

Schäfer, G., Cramer, T., Suske, G., Kemmner, W., Wiedenmann, B. and Höcker, M. (2003). Oxidative stress regulates Vascular Endothelial Growth Factor-A transcription through Sp1- and Sp3-dependent activation of two proximal GC-rich promoter elements. J. Biol. Chem. 278, 8190-8198.

Sapetschnig, A., Rischitor, G., Braun, H., Doll, A., Schergaut, M., Melchior, F. and Suske, G. (2002). Transcription factor Sp3 is silenced through SUMO modification by PIAS1. EMBO J. 21, 5206-5215.

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Kraus, A.C., Ferber, I., Bachmann, S.O., Specht, H., Wimmel, A., Gross, MW, Schlegel, J., Suske, G. and Schürmann, M. (2002). In vitro chemo- and radio-resistance in small cell lung cancer correlates with cell adhesion and constitutive activation of AKT and MAP kinase pathways. Oncogene 21, 8683-8695.

Chadjichristos, C., Ghayor, C., Herrouin, J.F., Ala-Kokko, L., Suske, G., Pujol, J.P. and Galera, P. (2002). Down-regulation of human type II collagen gene expression by transforming growth factor-beta 1 (TGF-beta 1) in articular chondrocytes involves SP3/SP1 ratio. J. Biol. Chem. 277, 43903-43917.

Cassel, T.N., Berg, T., Suske, G. and Nord, M. (2002). Synergistic transactivation of the differentiation dependent lung gene CCSP by the bZIP factor C/EBPalpha and the homeodomain factor Nkx2.1/TTF-1. J. Biol. Chem. 277, 36970-36977

Braun, H., Koop, R. Ertmer, A., Nacht, S. and Suske, G. (2001). Transcription factor Sp3 is regulated by acetylation. Nucleic Acids Res. 29, 4994-5000.

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Ghayor, C., Chadjichristos, C., Herrouin, J. F., Ala-Kokko, L., Suske, G., Pujol, J. P., and Galera, P. (2001). Sp3 represses the Sp1-mediated transactivation of the human COL2A1 gene in primary and dedifferentiated chondrocytes. J. Biol. Chem. 10, 36881-36895.

Göllner, H., Dani, C., Phillips, B., Philipsen, S., and Suske, G. (2001). Impaired ossification in mice lacking the transcription factor Sp3. Mech. Dev. 106, 77-83.

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Göllner, H., Bouwman, P., Mangold, M., Karis, A., Braun, H., Rohner, I., Del Rey, A., Besedovsky, H.-O., Meinhardt, A., van den Broek, M., Cutforth, T., Grosveld, F., Philipsen, S., and Suske G. (2001). Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene. Genes Cells 6, 691-700.

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Song, J., Mangold, M., Suske, G., Geltinger, C., Kanazawa, I., Sun, K., and Yokoyama, K. K. (2001). Characterization and promoter analysis of the mouse gene for transcription factor Sp4. Gene 264, 19-27.

Nord, M., Cassel, T. N., Braun, H., and Suske, G. (2000). Regulation of the Clara cell secretory protein/uteroglobin promoter in lung. Ann. N. Y. Acad. Sci. 923, 154-165.

Cassel, T. N., Suske, G., and Nord, M. (2000). C/EBP alpha and TTF-1 synergistically transactivate the Clara cell secretory protein gene. Ann. N. Y. Acad. Sci. 923, 300-302.

G. Suske (2000). Transient transfection of Schneider cells in the study of transcription factors. Methods Mol. Biol., 130, 175-187

R.J.P. Bouwman*, H. Göllner*, H.-P. Elsässer, G. Eckhoff, A. Karis, F.G. Grosveld, S. Philipsen and G. Suske (2000). Transcription factor Sp3 is essential for post-natal survival and late tooth development.
EMBO J. 19, 655-661. *Peter Bouwman and Heike Göllner contributed equally to this work.

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G. Suske (1999). The Sp-family of transcription factors. Gene 238, 291-300

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S. Philipsen and G. Suske (1999). A tale of three fingers: the family of mammalian Sp/XKLF transcription factors. Nucleic Acids Res. 27, 2991-3000

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H. Braun and G. Suske (1999). Vectors for Inducible Expression of Dual Epitope-Tagged Proteins in Insect Cells. BioTechniques 26, 1038-1042

H. Ding, A.M. Benotmane, G. Suske, D. Collen and A. Belayew (1999). Functional interactions between Sp1 or Sp3 and the helicase-like transcription factor mediate basal expression from the human PAI-1 gene. J. Biol. Chem. 274, 19573-19580

R. Ahlgreen, G. Suske, M.R. Waterman and J. Lund (1999). Role of Sp1 in cAMP-dependent transcriptional regulation of the bovine CYP11A gene. J. Biol. Chem. 274, 19422-19428

S. Müller, A. Maas, T.C. Islam, P. Sideras, G. Suske, S. Philipsen, K.G. Xanthopoulos, R.W. Hendriks and C.I.E.Smith, (1999). Synergistic activation of the human Btk promoter by transcription factors Sp1/3 and PU.1. Biochem. Biophys. Res. Comm., 259, 364-369

H. Braun and G. Suske (1998). Combinatorial action of HNF3- and Sp-family transcription factors in the activation of the rabbit uteroglobin/CC10 promoter. J. Biol. Chem. 273, 9821-9828.

B. Simon, F. Wattler, J. L. Merchant, K. Munch, H. J. Schutze, G. Suske and R. Arnold (1997). RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors. FEBS Lett. 411, 383-388.

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S. Liu, A. M. Borras, P. Liu, G. Suske and S. H. Speck (1997). Binding of the ubiquitous cellular transcription factors Sp1 and Sp3 to the ZI domains in the Epstein-Barr virus lytic switch BZLF1 gene promoter. Virology 228, 11-18.

J. Dennig, M. Beato and G. Suske (1996). An inhibitor domain in Sp3 regulates its glutamine-rich activation domains. EMBO J. 15, 5659-5667.

M. Kalff-Suske, J. Kunz, K.-H. Grzeschik and G. Suske (1996). Human Sp3 Transcriptional Regulator Gene (SP3) maps to chromosome 2q31. Genomics 37, 410-412.

D. Apt, R.M. Watts, G. Suske and H.-U. Bernard (1996). High Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter. Virology 224, 281-291.

Y. Liang, D. F. Robinson, J. Dennig, G. Suske and W. E. Fahl (1996)
Transcriptional regulation of the SIS/PDGF-B gene in human osteosarcoma cells by the Sp family of transcription factors. J. Biol. Chem. 271, 11792-11797.

G. Hagen, J. Dennig, A. Preiß, M. Beato and G. Suske (1995). Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3. J. Biol. Chem. 270, 24989-24994.

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M.J. Birnbaum, A.J. van Wijnen, P. Odgren, T.J. Last, G. Suske, G.S. Stein and J.L. Stein (1995). Sp1 trans-activation of cell-cycle regulated promoters is selectively repressed by Sp3. Biochemistry 34, 16503-16508.

J. Dennig, G. Hagen, M. Beato and G. Suske (1995). Members of the Sp transcription factor family control transcription from the uteroglobin promoter. J. Biol. Chem. 270, 12737-12744.

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B. Majello, P. De Luca, G. Suske and L. Lania (1995). Differential transcriptional regulation of c-myc promoter through the same DNA binding sites targeted by Sp1-like proteins. Oncogene 10, 1841-1848.

M. Kalff-Suske, J. Kunz, K.-H. Grzeschik and G. Suske (1995). Human Sp4 transcription factor gene (SP4) maps to chromosome 7p15. Genomics 26, 631-633

R. Dunkel, G. Vriend, M. Beato and G. Suske (1995). Progesterone binding to uteroglobin: Two alternative orientations of the ligand. Prot. Engng. 8, 71-79.

A. Sandmöller, R. Halter, G. Suske, D. Paul and M. Beato (1995). A transgenic mouse model for lung adenocarcinoma. Cell Growth Diff. 6, 97-103.

B. Majello, P. De Luca, G. Hagen, G. Suske and L. Lania (1994). Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1. Nucleic Acids Res. 22, 4914-4921.

G. Hagen, S. Müller, M. Beato and G. Suske (1994). Sp1-mediated transcriptional activation is repressed by Sp3. EMBO J. 13, 3843-3851.

A. Sandmöller, R. Halter, E. Gomez-La-Hoz, H.-J. Gröne, G. Suske, D. Paul and M. Beato (1994). The uteroglobin promoter targets expression of the SV40 T antigen to a variety of secretory epithelial cells in transgenic mice. Oncogene 9, 2805-2815.

L.K. Vogel, G. Suske, M. Beato, O. Norén and H. Sjöström (1993). Uteroglobin, an apically secreted protein of the uterine epithelium, is secreted non-polarized from MDCK cells and basolaterally from Caco-2 cells. FEBS Lett. 330, 293-296.

G. Hagen, S. Müller, M. Beato and G. Suske (1992). Cloning by recognition site screening of two novel GT box binding proteins: A family of Sp1 related genes. Nucleic Acids Res. 20, 5519-5525.

Peter, W., Dunkel, R., Stouten, P. F., Vriend, G., Beato, M., and Suske, G. (1992). Interchain cysteine bridges control entry of progesterone to the central cavity of the uteroglobin dimer. Protein Eng. 5, 351-359.

Suske, G., Lorenz, W., Klug, J., Gazdar, A. F., and Beato, M. (1992). Elements of the rabbit uteroglobin promoter mediating its transcription in epithelial cells from the endometrium and lung. Gene Expr. 2, 339-352.

Wolf, M., Klug, J., Hackenberg, R., Gessler, M., Grzeschik, K. H., Beato, M., and Suske, G. (1992). Human CC10, the homologue of rabbit uteroglobin: genomic cloning, chromosomal localization and expression in endometrial cell lines. Hum. Mol. Genet. 1, 371-378.

Peter, W., Brüller, H. J., Vriend, G., Beato, M., and Suske, G. (1991). Identification of residues essential for progesterone binding to uteroglobin by site-directed mutagenesis. J. Steroid Biochem. Mol. Biol. 38, 27-33.

Sandmöller, A., Voss, A. K., Hahn, J., Redemann-Fibi, B., Suske, G., and Beato, M. (1991). Cell-specific, developmentally and hormonally regulated expression of the rabbit uteroglobin transgene and the endogenous mouse uteroglobin gene in transgenic mice. Mech. Dev. 34, 57-67.

Helftenbein, G., Misseyanni, A., Hagen, G., Peter, W., Slater, E. P., Wiehle, R. D., Suske, G., and Beato, M. (1991). Expression of the uteroglobin promoter in epithelial cell lines from endometrium. Ann. N Y Acad. Sci. 622, 69-79.

Misseyanni, A., Klug, J., Suske, G., and Beato, M. (1991). Novel upstream elements and the TATA-box region mediate preferential transcription from the uteroglobin promoter in endometrial cells. Nucleic Acids Re. 19, 2849-2859.

Hagen, G., Wolf, M., Katyal, S. L., Singh, G., Beato, M., and Suske, G. (1990). Tissue-specific expression, hormonal regulation and 5’-flanking gene region of the rat Clara cell 10 kDa protein: comparison to rabbit uteroglobin. Nucleic Acids Res. 18, 2939-2946.

Slater, E. P., Redeuihl, G., Theis, K., Suske, G., and Beato, M. (1990). The uteroglobin promoter contains a noncanonical estrogen responsive element. Mol. Endocrinol. 4, 604-610.

Voss, A. K., Sandmoller, A., Suske, G., Strojek, R. M., Beato, M., and Hahn, J. (1990). A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection. Theriogenology 34, 813-824.

Peter, W., Beato, M., and Suske, G. (1989). Recombinant rabbit uteroglobin expressed at high levels in E. coli forms stable dimers and binds progesterone. Protein Eng. 3, 61-66.

Scheidereit, C., von der Ahe, D., Cato, A. C., Wenz, M., Suske, G., Carlson, C., Bosshard, H., Westphal, H. M., and Beato, M. (1989). Protein-DNA interactions at steroid hormone regulated genes. Endocr. Res. 15, 417-440.

Suske, G., Gross, B., and Beato, M. (1989). Non-radioactive method to visualize specific DNA-protein interactions in the band shift assay. Nucleic Acids Res. 17, 4405.

Suske, G., Enders, C., Schlerf, A., and Kadenbach, B. (1988). Organization and nucleotide sequence of two chromosomal genes for rat cytochrome c oxidase subunit VIc: a structural and a processed gene. Nucleic Acids Res. DNA 7, 163-171.

Suske, G., Mengel, T., Cordingley, M., and Kadenbach, B. (1987). Molecular cloning and further characterization of cDNAs for rat nuclear-encoded cytochrome c oxidase subunits VIc and VIII. Eur. J. Biochem. 168, 233-237.

Suske, G., Westphal, H. M., and Beato, M. (1986). Mechanism of gene regulation by steroid hormones. J. Steroid Biochem. 24, 19-24.

Scheidereit C, Krauter P, von der Ahe D, Janich S, Rabenau O, Cato AC, Suske G, Westphal HM, Beato M. (1986). Mechanism of gene regulation by steroid hormones. J. Steroid Biochem. 24, 19-24.

Suske, G., Wenz, M., Cato, A. C., and Beato, M. (1983). The uteroglobin gene region: hormonal regulation, repetitive elements and complete nucleotide sequence of the gene. Nucleic Acids Res. 11, 2257-2271.

Menne, C., Suske, G., Arnemann, J., Wenz, M., Cato, A. C., and Beato, M. (1982). Isolation and structure of the gene for the progesterone-inducible protein uteroglobin. Proc. Natl. Acad. Sci. U S A 79, 4853-4857.

Suske, G., Menne, C., Cato, A., Wenz, M., and Beato, M. (1982). Structure and regulated expression of the uteroglobin gene. Prog. Clin. Biol. Res. 102 pt A, 139-146.

Suske G., Wagner W. and Follmann H. (1979). NADPH-dependent thioredoxin reductase and a new thioredoxin from wheat. Z. Naturforsch 34c, 214-221.