C2: Significance of ATP-dependent chromatin remodeler for the NFkB action

The ATP-dependent chromatin remodeler Mi-2 has recently been demonstrated to play a role during the activation of pro- inflammatory genes. It is not known if Mi-2 related enzymes of the CHD family or Mi-2 associated proteins also contribute to the activation of the pro-inflammatory genes. We will investigate, how RNA interference-mediated depletion of these factors influences the activation of pro-inflammatory genes. We will use LPS and TNFα and stimulate cells via Toll-like receptors (TLRs). First, we will determine the effects on expression of selected established pro-inflammatory genes. At a later stage, we will extend our study to the genomewide level by using microarray technology. Theses analysis will allow us to define the roles of CHD ATPases and Mi-2 associated proteins during inflammation.